Clinical significance of lymphocyte subsets and platelet specific antibodies in patients with idiopathic thrombocytopenic purpura
10.3760/cma.j.issn.1673-4904.2010.28.011
- VernacularTitle:特发性血小板减少性紫癜患者外周血淋巴细胞亚群和血小板特异性抗体检测的临床意义
- Author:
Lingling SUN
;
Bin JI
;
Yanli MENG
- Publication Type:Journal Article
- Keywords:
Purpura,thrombocytopenic,idiopathic;
Lymphocyte subsets;
Antibodies
- From:
Chinese Journal of Postgraduates of Medicine
2010;33(28):32-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the changes of lymphocyte subsets and anti-GP Ⅱ b/Ⅲa,anti-GP Ⅰ b/Ⅸ specific antibodies in patients with idiopathic thrombocytopenic purpura(ITP),and discuss the related factors involved in pathogenesis of ITP.Methods The changes of lymphocyte subsets CD3+,CD4+,CD8+,CD4+CD8+,CD19+ in 72 patients with ITP (ITP group) and 50 healthy controls (control group) were measured by flow cytometry (FCM).Expression of anti-GP Ⅱ b/Ⅲ a,anti-GP Ⅰ b/Ⅸ specific antibodies were tested by monoclonal antibody immobilization of platelet antigens assay (modified MAIPA).Results Optical density of anti-GP Ⅱ b/Ⅲ a,anti-GP Ⅰ b/Ⅸ specific antibodies in ITP group was much higher than that in control group (P ＜0.05).In changes of lymphocyte subsets,the number of the CD3+,CD4+,CD4+/CD8+ in ITP group were obviously lower than those in control group (P ＜0.01),but CD8+ and CD19+ were much higher than those in control group(P ＜0.01).Conclusions Expression of lymphocyte subsets and platelet specific antibodies can reflect the pathogenesis of ITP well.The abnormal function and ratio of lymphocyte subsets,as well as the immunologic mechanism disorder of lymphocytes play important roles in the pathogenesis of ITP.
