Effect of pioglitazone on PPARγ mRNA expression in rats with acute necrotizing pancreatitis
10.3760/cma.j.issn.1674-1935.2010.05.016
- VernacularTitle:吡格列酮对急性坏死性胰腺炎大鼠胰腺PPARγ mRNA表达的影响
- Author:
Ping XU
;
Qinghua LI
;
Jing WANG
;
Kai XU
;
Jingping JIANG
;
Lingquan CHEN
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Peroxisome proliferator-activated receptors;
Pioglitazone
- From:
Chinese Journal of Pancreatology
2010;10(5):352-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of pioglitazone on peroxisome proliferators activated receptors (PPARγ) mRNA expression in rats with acute necrotizing pancreatitis (ANP). Methods 54 rats were randomly divided into sham group, ANP group, and pioglitazone group. ANP was induced by the retrograde injection of sodium taurocholate into pancreatic duct. The levels of serum amylase at 3 h, 6 h, and 12 h after ANP induction were determined and pancreatic pathological scores were measured, and the expression of pancreatic PPARγ mRNA was determined by RT-PCR. Results The levels of serum amylase and pancreatic pathological scores at 6 h in ANP group were (7171 ± 1636) U/L and 13.00 ±2.36; which were significantly higher than those in sham group [ (523 ± 166)U/L and 1.67 ± 234, P < 0. 01 ]. While the expressions of PPARγ mRNA were weakly expressed in ANP gyoup and sharm group(0. 18 ± 0.05 vs 0. 22 ±0. 03, P > 0. 05). The levels of serum amylase and pancreatic pathological scores at 6 h in pioglitazone group were (4504 ± 1901 )U/L and 9.00 ±0.89, which were significantly lower than those in ANP group (P <0. 05).While the expressions of PPARγ mRNA was higher than that in ANP group (0.56 ±0.05 vs 0. 18 ±0.05, P <0.05). Conclusions The protective role of pioglitazone on ANP rats was closely correlated with up-regulation of PPARγgene expression.
