Effect of high-density lipoprotein on interleukin-8 secretion in 3T3-L1 adipocytes
10.3760/cma.j.issn.1000-6699.2010.10.016
- VernacularTitle:高密度脂蛋白对3T3-L1脂肪细胞分泌白细胞介素8的影响
- Author:
Qiaoqing ZHONG
;
Shuiping ZHAO
;
Xing WANG
;
Bilian YU
;
Jing DONG
;
Xiangzhu XIE
;
Zhihong WU
- Publication Type:Journal Article
- Keywords:
High-density lipoprotein;
Adipocytes;
Interleukin-8;
PPARγ
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(10):888-890
- CountryChina
- Language:Chinese
-
Abstract:
3T3-L1 adipocytes were cultured with various concentrations of high-density lipoprotein ( HDL, 0, 10, 50, and 100 μg/ml ) for 16 h and with lipopolysaccharide ( LPS, 100 ng/ml ) for another 6 h. Interleukin-8 in the medium was determined by ELISA, and PPAR-γ mRNA expression by reverse transacription polymerase chain reaction (RT-PCR). Interleukin-8 levels were increased in LPS-treated cells ( P＜0.05 ), but decreased in HDL-treated cells in the dose-dependent manner. PPARγ mRNA expressions were increased in HDL-treated groups than those treated only with LPS. These results suggested HDL may decrease interleukin-8 secretion via up-regulating PPARγ expression in adipocytes.
