Biological safety of acellular spinal cord scaffold: an in vitro study

VernacularTitle:脱细胞脊髓支架生物安全性的体外研究
Author: Shuzhang GUO ; Xianjun REN ; Tao JIANG
Publication Type:Journal Article
Keywords: Spinal injuries; Biocompatible material; Acellular spinal cord
From: Chinese Journal of Trauma 2010;26(10):930-933
CountryChina
Language:Chinese
Abstract: Objective To evaluate the in vitro biological safety of acellular spinal cord scaffold so as to provide theoretical basis for constructing the ideal tissue engineering scaffold of spinal cord.Methods A piece of thoracic spinal cord for 2 cm removed from SD rats was harvested and then was treated by freezing and thawing and chemical extraction with 3% sodium deoxyeholate and 1 KU/ml DNaseI and RNaseA. Gross observation and histological examination of the acellular spinal cord scaffold were carried out to learn the condition of the extracellular matrix scaffold. The biological safety of the acellular spinal cord scaffold was evaluated. Results In cross section, network of the extracellular matrix was presented in the scaffold. The cells, myelin and axons disappeared after the spinal cord was treated with sodium deoxycholate, DNaseI and RNaseA. Typical network of empty tubes were viewed in longitudinal sections. General toxic reaction, pyrogen test, hemolysis test and cytotoxicity test were conforming to the standard of materials. Conclusion As neotype tissue engineering material, the acellular spinal cord scaffold has satisfactory biological safety.