Effect of implantation of bone marrow mononuclear cells on ischemic-type intrahepatic biliary lesion in rabbits
10.3760/cma.j.issn.1007-8118.2010.10.021
- VernacularTitle:自体骨髓单核细胞移植对兔肝内缺血型胆道病变的影响
- Author:
Zhaowei QU
;
Qinsong SHENG
;
Dazhi CHEN
;
Ren LANG
;
Qiang HE
;
Fei PAN
;
Xiaosheng ZHANG
- Publication Type:Journal Article
- Keywords:
Transplantation;
Bone marrow mononuclear cell;
Intrahepatic bile duct;
Ischemia
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(10):781-784
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of implantation of bone marrow mononuclear cells (BM-MNCs)on neovascularization and ischemic-type intrahepatic biliary lesion in rabbits.Methods The animals were divided into the sham-operation group, experimental model group and BM-MNCs implantation group with 10 rabbits in each. The animal model of ischemic-type intrahepatic biliary lesion was established by clamping the hepatic artery and common bile duct. The BM-MNCswere isolated from the tibial plateau by means of density gradient centrifugation and were implanted through the common hepatic artery. Changes of some biochemical markers such as ALT, AST, ALP,γ-GT, TBIL and DBIL etc. were detected. In 4 weeks after operation, the cholangiography, histopathological manifestation, differentiation of BM-MNCs, and microvessel density were observed.Results At each observation time, the degree of change of biochemical markers in group C was lower than that in group B. The engrafted cells could differentiate into vascular endothelial cells. The intrahepatic biliary lesion of group B was severer than that of group C but had fewer new capillary blood vessels around it. Conclusion The implantation of BM-MNCs can promote neovascularization and increase blood supply to the ischemic bile duct to diminish or prevent ischemic-type intrahepatic biliary lesion.
