Effects of neural stem cell transplantation from GFP transgenic mice on rat neurocognitive function and NGF expression after brain trauma
10.3760/cma.j.issn.1674-6554.2010.10.005
- VernacularTitle:荧光蛋白转基因小鼠神经干细胞移植对脑损伤大鼠认知功能及神经生长因子表达的影响
- Author:
Ke MAO
;
Hao LI
;
Jing CHEN
;
Hong ZHU
;
Chao YOU
;
Guoping LI
- Publication Type:Journal Article
- Keywords:
Contusion brain injury;
Neural stem cells;
Neurological function;
Nerve growth factor
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(10):876-878
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish contusion brain injury model in rats, and investigate the efficacy of intravenous administration of neural stem cells(NSC) on posttraumatic neurocognitive function recovery and NGF expression in rats. Methods Cerebral contusion model in motor-sensory cortex of the right parietal cortex in rat was established by a 50 g-weight hammer falling respectively from 30 cm height along guide stick to impact collision pole by improved trauma device for model of contusion brain injury based on Feeney method. And the NSC isolated from GFP transgenic mice were injected intravenously via the tail vein 24 h after the brain trauma, and 1 week later neurocognitive function scores and NGF immunostaining were performed to explore the efficacy of NSC transplant. Results The NSCs from the GFP transgenic mice gathered at the injury site 1 weeks after transplants.Neurocognitive function scores and NGF-positive cells measurement(226 ±27,23 ±4 ) in the treatment group revealed significant increase than in the brain trauma group(300 ±36;15 ±3 )(P＜0.05). Conclusion The intravenous NSC injection in rats can survive and migrate to the injured brain region and promote the post-injury neurocognitive function restoration. The increase of NGF expression may underline one of most important mechanisms in NSC treatment' s rats after brain injury.
