Insulin promotes rhesus retinal vascular endothelial cells angiogenesis via VEGF-A/VEGFR2 pathway
10.3760/cma.j.issn.1000-6699.2010.10.017
- VernacularTitle:胰岛素经VEGF-A/VEGFR2途径促进恒河猴视网膜血管内皮细胞的血管新生
- Author:
Saijun ZHOU
;
Song CHEN
;
Pei YU
;
Demin YU
- Publication Type:Journal Article
- Keywords:
Insulin;
Rhesus retinal vascular endothelial cell line RF/6A;
Vascular endothelial growth factor;
Vascular endothelial growth factor receptors;
Angiogenesis
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(10):891-893
- CountryChina
- Language:Chinese
-
Abstract:
Rhesus retinal vascular endothelial cell line RF/6A cells were treated with human insulin. Cell proliferation, migration, and lumen formation, as well as the expressions of vascular endothelial growth factor-A ( VEGF-A ), VEGF-A receptors, and phosphorylated receptors were measured. Insulin promoted RF/6A cell proliferation, migration, and lumen formation ( all P＜0. 01 ). Insulin increased the expression of VEGF-A mRNA and improved its protein activity ( all P＜0. 05 ), and promoted the expression of VEGFR2 mRNA and its phosphorylation ( both P＜0. 01 ). There was no significant difference in the expression of VEGFR1 mRNA among the groups ( P＞0. 05 ).
