Inhibition of colorectal cancer cell adhesion by cimetidine
10.3760/cma.j.issn.1673-422X.2010.10.025
- VernacularTitle:西咪替丁抑制结直肠癌细胞黏附的研究
- Author:
Ping ZHOU
;
Shuqian HE
;
Yuxia CHENG
;
Qing SUN
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Endothelial cells;
Neoplasms metastasis;
Cimetidine
- From:
Journal of International Oncology
2010;37(10):795-798
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Cimetidine on the adhesion between colorectal cancer LOVO cells and endothelial ECV304 cells;and study whether Cimetidine can inhibit the expression of E-selectin in ECV304 cells. Methods Cellular uptake of rose Bengal stain was used to measure the adhesion between LOVO cells and ECV304 cells. Enzyme-linked immunosorbent assay(ELISA)and flow cytometry (FCM, indirect fluorescence staining and labeling)were used to detect the expression of E-selectin. Results ECV304 cells were exposed to different concentrations of Cimetidine. Both the adhesion between LOVO cells and ECV304 cells and the levels of E-selectin significantly decreased with increasing concentration of Cimetidine(P =0. 001 and 0. 001 respectively). Conclusion Cimetidine can inhibit the adhesion of human colorectal cancer LOVO cells on endothelial ECV304 cells by blocking E-selectin expression. Our observations indicated a potential of anti-adhesion therapy using Cimetidine in cancer treatment.
