Effects of the recipe for activating blood circulation and nourishing qi on expression of mitochondrial ribosomal protein L51 in CVB3 infection model in rat cardiac myocytes
10.3760/cma.j.issn.1673-4246.2010.06.001
- VernacularTitle:益气活血中药复方对CVB3乳鼠心肌细胞感染模型线粒体核糖体蛋白L51表达的影响
- Author:
Mingxue ZHANG
;
Yumei ZHANG
;
Wei HE
;
Honghua CHE
;
Ping GU
- Publication Type:Journal Article
- Keywords:
Viral myocarditis;
Recipe for activating blood circulation and nourishing qi;
Suppression subtractive hybridization (SSH);
Mitochondrial ribosomal protein L51 (MRPL51)
- From:
International Journal of Traditional Chinese Medicine
2010;32(6):485-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of the recipe for activating blood circulation and nourishing qi on expression of mitochondrial ribosomal protein L51 (MRPL51) in CVB3 infection model in rat cardiac myocytes, to reveal the pathogenesis of CVB3 myocarditis in the genetic level, to explore the therapeutic mechanism of the recipe for activating blood circulation and nourishing qi on CVB3 myocarditis, and to confirm the validity of the recipe for activating blood circulation and nourishing qi on CVB3 myocarditis. Methods After establishing CVB3 infection model and treatment model with recipe for activating blood circulation and nourishing qi by culturing neonatal rat myocardial cells, a modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between two model groups. These results were further verified by fluorescence RT-PCR. Results The results of SSH showed that gene expression of the treatment group was lower than that of the CVB3 infection group. The results of fluorescent RT-PCR which agreed with that of SSH displayed the threshold cycle number (Ct) in the treatment group was higher than the virus group. Conclusion Up-regulation of MRPL51 might be one of the pathogenesis of CVB3 myocarditis. The recipe for activating blood circulation and nourishing qi could treat viral myocarditis by regulating the expression of MRPL51.
