Effects of propofol on activation of NF-κB in polymorphonuclear neutrophils in rats with lipopolysaccharide-induced acute lung injury
10.3760/cma.j.issn.0254-1416.2010.07.030
- VernacularTitle:异丙酚对内毒素性急性肺损伤大鼠中性粒细胞NF-κB活化的影响
- Author:
Sha LI
;
Yan ZHANG
;
Sheng PENG
- Publication Type:Journal Article
- Keywords:
Propofol;
NF-κB;
Neutrophils;
Endotoxemia;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2010;30(7):862-864
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on activation of NF-κB in polymorphonuclear neutrophils (PMNs) in rats with LPS-induced acute lung injury (ALI). Methods Sixty healthy SD rats of both sexes, aged 3 months, weighing 250-350 g, were randomly divided into 5 groups (n = 12 each):control group (group C), ALI group and 3 different dose of propofol groups (group P1, P2, P3). The animals were anesthetized with intraperitaneal 3% pentobarbital sodium 40 mg/kg. LPS 5 mg/kg was injected via femoral vein in group ALI.Propofol 5, 10 and 15 mg· kg- 1· h- 1 was infused intravenously over 2 h immeliately after injection of LPS 5 ng/kg through femoral vein in group P1, P2 and P3 respectivey. In group C normal saline 10 ml was injected via femoral vein instead. All rats were killed by exsanguination at the end of infusion of propofol. The right lung was removed for microscopic examination. The morphologic changes were scored 0-3 (0 = normal, 3 = severe morphologic changes). Blood samples were collected from carotid artery for determination of the expression of total NF-κB and activated NF-κB in PMNs by Western blot. Results Compared with group C, morphologic change scores and activated NF-κB expression in PMNs were significantly increased in group ALI, P1 and P2, and morphologic change scores increased in group P3. Morphologic change scores in group P1 and P2 and activated NF-κB expression in PMNs in group P1, P2 and P3 were significantly decreased compared with those in group ALl. Morphologic change scores and activated NF-κB expression in PMNs were decreased gradually in group P1, P2 and P3 . There was no significant difference in total NF-κB expression in PMNs among all groups. Conclusion Propofol can attenuate ALI induced by LPS through inhibition of the activation of NF-κB in PMNs in rats.
