Effect of propofol pretreatment on hypoxia-induced apoptosis of alveolar epithelial type Ⅱ cells in fetal rats
10.3760/cma.j.issn.0254-1416.2010.07.031
- VernacularTitle:异丙酚预先给药对缺氧诱导胎鼠肺泡Ⅱ型上皮细胞凋亡的影响
- Author:
Xingying HE
;
Xueyin SHI
;
Hongbin YUAN
;
Haitao XU
;
Shuangqiong ZHOU
;
Wenyun XU
- Publication Type:Journal Article
- Keywords:
Propofol;
Epithelial cells;
Pulmonary alveoli;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2010;30(7):865-867
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propofol pretreatment on hypoxia-induced apoptosis of alveolar epithelial type Ⅱ (AE Ⅱ) cells in fetal rats. Methods Primary cultured AE Ⅱ cells isolated from fetal rats were seeded in 96-well plates (1 × 106/L, 180 μl/well) and randomly assigned to one of 3 groups (n = 72each):normal control group (group C), hypoxia group (group H) and propofol-hypoxia group (group P-H).Group H and P-H were exposed to hypoxia (5% O2). In group P-H, propofol (final concentration 5 μ mol/L) was added 1 h prior to hypoxia (5% O2). The apoptotic rate and expression of hypoxia-inducible factor (HIF)-1αmRNA, Bnip3L mRNA, HIF-1α protein and Bnip3L protein were determined at 3, 12, 24 and 48 h of hypoxia.Results The apoptotic rate and expression of HIF-1α mRNA, Bnip3L mRNA, HIF-lα protein and Bnip3L protein were significantly up-regulated in group H compared with group C (P ＜ 0.05). Propofol pretreatment could significantly inhibit the hypoxia-induced changes mentioned above (P ＜ 0.05). Conclusion Propofol pretreatment can inhibit hypoxia-induced apoptosis of AE Ⅱ cells, and the mechanism is related to inhibition of HIF-1αactivation and down-regulation of Bnip3L expression in fetal rats.
