Role of renal cell apoptosis in acute kidney injury induced by sepsis in mice
10.3760/cma.j.issn.0254-1416.2010.08.033
- VernacularTitle:肾组织细胞凋亡在脓毒症诱发小鼠急性肾损伤中的作用
- Author:
Lixia LIU
;
Yong LI
;
Zhenjie HU
;
Liqiao FAN
;
Bibo TAN
;
Yan HUO
;
Jie SU
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Sepsis;
Kidney
- From:
Chinese Journal of Anesthesiology
2010;30(8):1006-1008
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of renal cell apoptosis in acute kidney injury (AKI) induced by sepsis in mice. Methods Forty-five male C57BL/6 mice were randomly assigned into 3 groups ( n = 15 each):sham operation group (group S), cecum ligation and puncture group (group CLP) and CLP + caspase-3 inhibitor Ac-DEVD-CHO group (group CI). Intra-abdominal infection was induced by CLP. Ac-DEVD-CHO 4 μg/g was infused subcutaneously 30 min before CLP in group CI. Five mice in each group were sacrificed after collection of blood samples at 6, 12 and 24 h after CLP. The levels of serum blood urea nitrogen (BUN) and creatinine (Cr)were detected. The apoptosis rate and expression of caspase-3 protein and caspase-3 mRNA were determined.Pathological changes in renal tissues were observed with light microscope. Results The serum BUN and Cr concentratiors, apoptosis rate and expression of caspase-3 mRNA and caspase-3 protein were significantly higher in group CLP than in group S, but lower in group CI than in group CLP ( P ＜ 0.05). Light microscopic examination showed that the pathologic changes induced by Ac-DEVD-CHO were less severe in group CI than in group CLP.Conclusion The renal cell apoptosis is one of the mechanism of AKI induced by sepsis.
