Expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice subject to skin transplantation
10.3760/cma.j.issn.0254-1785.2010.11.012
- VernacularTitle:IL-23、IL-23 mRNA在皮肤移植受鼠外周血及移植物中的表达
- Author:
Guosheng DU
;
Bingyi SHI
;
Dehua ZHENG
;
Jiyong SONG
;
Zhidong ZHU
;
Hongtao CUI
- Publication Type:Journal Article
- Keywords:
Acute rejection;
IL-23;
mRNA;
Spleen cells
- From:
Chinese Journal of Organ Transplantation
2010;31(11):683-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice receiving skin transplantation under different immune states. Methods Mice skin allograft models were established and divided into 3 groups: synergeneic transplant group (BALB/c→BALB/c), allogeneic transplant group (C57BL/6→BALB/c), donor spleen cells infusion group (C57BL/6→BALB/c). Peripheral blood plasma concentration of IL-23 was measured by ELISA. RT-PCR was used to detect the expression of IL-23 mRNA in the skin allograft. Results There was no significant difference in the IL-23 and IL-23 mRNA expression among all three groups one day after skin transplantation (P＞0. 05). On the day 3, 5, and 7 after skin transplantation, there was significant difference in the IL-23 and IL-23 mRNA expression levels between synergeneic transplant group, donor spleen cells infusion group and allogeneic transplant group (P ＜ 0. 01 ), but there was no significant difference between synergeneic transplant group and donor spleen cells infusion group (P＞0. 05). Conclusion The high expression levels of IL-23 and IL-23 mRNA were detected when early acute rejection took place in recipient mice. IL-23 could serve as a predictable and prognostic marker for the acute rejection. Infusion of donor spleen cells can significantly prolong the allograft survival.
