Effects of Triptolide on expression of toll-like receptor 4 in renal ischemia-reperfusion injury
10.3760/cma.j.issn.0254-1785.2010.11.014
- VernacularTitle:雷公藤内酯醇对大鼠肾缺血再灌注损伤时Toll样受体4表达的影响
- Author:
Jiangqiao ZHOU
;
Hui CHEN
;
Zhiyuan CHEN
;
Xiuheng LIU
;
Yunfei HU
;
Hengcheng ZHU
;
Minghuan GE
- Publication Type:Journal Article
- Keywords:
Triptolide;
Ischemia/reperfusion;
TLR4
- From:
Chinese Journal of Organ Transplantation
2010;31(11):688-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Triptolide on the expression of toll-like receptor 4 (TLR4) in renal ischemia/reperfusion (I/R) injury in rats. Methods A renal I/R model was established. Rats were randomly separated into the following experimental groups. Group 1, shamoperated control (n = 15) : rats were subjected to surgical manipulation, without the induction of renal ischemia. Group 2, I/R (n = 18): rats were subjected to left renal ischemia for 45 min followed by reperfusion. Group 3, TRI + I/R (n = 18): Before the I/R procedure (as in group 2), rats were intraperitoneally injected with TRI (0.4 mg/kg), once every day, three times. Rats were killed at the 1st, 3rd, and 5th day after I/R injury. The parameters of renal function were determined by autobiochemical analyzer. The expression of TLR4 was detected by RT-PCR and Western blotting. Results As comparedwith the sham-operated control group, serum BUN and Cr levels were significantly increased in the rats undergoing I/R procedure at the 1st, 3rd, and 5th day (P＜0. 01). After the treatment with TRI, the levels of BUN and Cr and the expression of TLR4 in the renal tissues were significantly decreased (P＜0. 05). Conclusion TRI could relieve renal I/R injury in rats by inhibiting the TLR4 expression.
