Role of protein kinase C in induction of vascular endothelial growth factor secretion by isoflurane in primary cultured rat cardiomyocytes
10.3760/cma.j.issn.0254-1416.2010.08.009
- VernacularTitle:蛋白激酶C在异氟醚诱导原代培养大鼠心肌细胞分泌血管内皮生长因子中的作用
- Author:
Zhigang LIU
;
Tao LUO
;
Yongfang LIU
;
Jinjin XU
;
Xiangdong CHEN
;
Zhongyuan XIA
;
Haosheng BI
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Myocytes,cardiac;
Protein kinase C;
Vascular endothelial growth factors
- From:
Chinese Journal of Anesthesiology
2010;30(8):922-924
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of protein kinase C (PKC) in induction of vascular endothelial growth factor (VEGF) secretion by isoflurane in primary cultured rat cardiomyocytes. Methods Primary cultured neonatal rat cardiomyocytes were randomly divided into 6 groups ( n = 6 each): control group (group C), 3 different concentration isoflurane groups (group Ⅰ1-3 ), PKC inhibitor calphostin C group (group P), and PKC inhibitor + isoflurane group (group PI). The cells were exposed to 0.7%, 1.4% and 2.1% isoflurane for6 h in group Ⅰ1-3 respectivly. Calphostin C was added to the culture medium with a final concentration of 50 nmol/L in group P. Calphostin C was added to the culture medium with a final concentration of 50 nmol/L, then the cells were exposed to 1.4% isoflurane for 6 h in group PI. VEGF concentrations and expression of PKC isoforms were determined by ELISA and Western blot respectively. Results Compared with group C, the VEGF concentration was significantly increased in group Ⅰ2 and Ⅰ3, and PKCε expression was down-regulated in the cytoplasm while upregulated in the cytomembrane in group Ⅰ2 ( P ＜ 0.01 ), but no significant change was found in the parameters mentioned above in group Ⅰ2 ( P ＞ 0.05). PKCα, PKCδ and PKCζ expression was significantly higher in the cytoplasm than in the cytomembrane in group C and Ⅰ2. VEGF concentrations were gradually increased with the increase in isoflurane concentrations ( P ＜ 0.05). VEGF concentrations were significantly lower in group PI than in Ⅰ2 ( P ＜0.05) .Conclusion Isoflurane induces VEGF secretion in primary cultured rat cardiomyocytes through translocation of PKCε from the cytoplasm to the cytomembrane, suggesting that it is a mechanism of the cardioprotective effects of isoflurane.
