The inhibitive effects of the protein active composition of Scorpio on apoptosis of L1210 tumor cells
10.3760/cma.j.issn.1673-4246.2010.06.003
- VernacularTitle:全蝎蛋白药效组分对L1210肿瘤细胞凋亡的影响
- Author:
Jingjuan WANG
;
Guijun ZHANG
;
Mingxia WU
;
Jingfan YANG
;
Qiyu LI
- Publication Type:Journal Article
- Keywords:
Protein active composition of Scorpio;
L1210 cells;
Apoptosis;
Evaluation of biological effect
- From:
International Journal of Traditional Chinese Medicine
2010;32(6):488-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of protein active composition of Scorpio on apoptosis of L1210 tumor cells for the purpose of establishing the quality evaluation method of biological effect of Scorpio. Methods L1210 cells were examined by trypan blue exclusion. The proliferation of cells was determined by improved MTT assay. Flow cytometry was performed to analyze cell apoptosis with propidium iodide (PI). Results When the concentration of protein active composition of Scorpio exceeded or equaled 37 mg/ml, the coefficient correlation of the growth inhibiting curve of L1210 cells was 0.9357, and IC50 was 175 mg/ml. The excellence time was 0 to 48 hours. When the concentration of protein active composition of Scorpio exceeded or equaled 9.25 mg/ml, the apoptosis ratio of L1210 cells was raised significantly.Conclusion The protein active composition of Scorpio might promote the apoptosis and restrain the proliferation of L1210 cells. The value of anti-tumor biological effect of the protein active composition of Scorpio was 9.25 ～ 175 mg/ml. This value may be one of the indexes for quality evaluation of biological effect of Scorpio.
