Acute toxicity of intravenous isoflurane in Beagles

VernacularTitle:Beagle犬静脉注射乳化异氟醚的急性毒性
Author: Aijie LIU ; Wensheng ZHANG ; Hanxiang MA ; Xiaolin YANG ; Zongbin YANG ; Yan RAO ; Nanfu LUO ; Jin LIU
Publication Type:Journal Article
Keywords: Isoflurane; Acute toxicity tests; Maximum tolerated dose
From: Chinese Journal of Anesthesiology 2010;30(7):772-773
CountryChina
Language:Chinese
Abstract: Objective To investigate the acute toxicity of intravenous isoflurane in Beagles.Methods Six healthy adult Beagles of both sexes aged 6-8 months weighing 6-8 kg were used in this study.Isoflurane injectio (120 mg/ml) in 30% hpid emulsion was injected intravenously. Femoral artery was cannulated for direct BP monitoring.ECG was continuously monitored.The maximal tolerance dose (MTD) and approximate lethal dose (ALD) were determined by up-and-down technique. The initial dose was 3.0 ml/kg. The dose was decreased/increased by 0.3 ml/kg if the previous animal died/survived.The survived dogs were observed for 2 weeks.Autopsy and histopathological examination were performed on all dead Beagles.Results The ALD and MTD of intravenous isoflurane were 252 and 216 mg/kg. Autopsy and histopathological examination did not show any abnormality.Conclusion Cardiopulmonary depression is the main manifestation of the acute toxicity of intravenous isoflurane in Beagles.