Effect of isoflurane on levels of PKA and PKC in hippocampus in rats
10.3760/cma.j.issn.0254-1416.2010.07.003
- VernacularTitle:异氟醚对大鼠海马蛋白激酶A和蛋白激酶C水平的影响
- Author:
Qing ZHANG
;
Guanyu LU
;
Zhipeng XU
;
Chunsheng FENG
;
Yun WANG
;
Anshi WU
;
Yun YUE
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Cyclic AMP-dependent protein kinases;
Protein kinase C;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2010;30(7):774-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of isoflurane on the levels of protein kinase A (PKA) and protein kinase C (PKC) in hippocampus in rats. Methods Thirty-six 3-month-old male SD rats weighing 180-220 g were randomly divided into 3 groups ( n = 12 each): group Ⅰ underwent the cognitive function test without being pretreated with isoflurane inhalation (group C); group Ⅱ and Ⅲ inhaled 1.2% isoflurane for 4 h and underwent the cognitive function test 2 days and 2 weeks later respectively (group Ⅰso1,Iso2). Morris water maze was used to assess the cognitive function and the escape latency was recorded. The animals were killed immediately after the test.The hippocampus was isolated for determination of the expression and activities of PKA and PKC.Results The escape latency was significantly longer in group Ⅲ than in group Ⅰ.The expression of PKA and PKC was significantly down-regulated and the activities of PKA and PKC were significantly decreased in group Ⅱand Ⅲ as compared with group Ⅰ . There was no significant difference in the expression and activities of PKA and PKC between group Ⅱ and Ⅲ . Conclusion Four hour 1.2% isoflurane inhalation can decrease cognitive function by inhibiting the levels of PKA and PKC in hippocampus.
