Effects of inhalation of different concentrations of isoflurane on hippocampal cytochrome c expression in aged rats
10.3760/cma.j.issn.0254-1416.2010.07.006
- VernacularTitle:吸入不同浓度异氟醚对老龄大鼠海马细胞色素c表达的影响
- Author:
Tieshan LUO
;
Shengchi SHI
;
Peng LI
;
Bin YI
;
Guocai TAO
- Publication Type:Journal Article
- Keywords:
Isoflurance;
Cytochromes c;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2010;30(7):784-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of inhalation of different concentrations of isoflurane on the expression of cytochrome c ( Cyt c) in hippocampus in aged rats.Methods Sixty-three aged male SD rats (20 months) weighing 500-600 g were randomly divided into 3 groups(n=21each):control group inhaling 30%O2 for 2h (group C) and 2 isoflurane groups anesthetized with 0.75 % and 1.5 % isoflurane in 30 % O2 for 2 h respectively (group Ⅰ1 and Ⅰ2 ).Arterial blood samples were obtained from 5 rats at 30 min, 1 and 2 h of anesthesia for blood gas analysis. Eight animals were killed at 24 h after anesthesia in each group.Their hippocampi were immediately removed for determination of Gyt c expression by immuno-histochemistry and Western blot analysis.Cognitive function was assessed by Morris water maze test the day before experiment and once a day for 6 consecutive days starting from the 1st postoperative day.Results The Cyt c expression in hippocampus was significantly increased in Ⅰ1 and Ⅰ2 groups in a concentration-dependent manner as compared with group C.The escape latency was significantly prolonged and the frequency of crossing the original platform and the duration of staying at the original platform quadrant were decreased in group Ⅰ1 and Ⅰ2 compared with group C.Conclusion Inhalation of isoflurane anesthesia can decrease cognitive function through up-regulating the Gyt c expression in hippocampus in aged rats.
