Effects of pentoxifylline on expression of uncoupling protein 2 of hepatic mitochondria in rats with nonalcoholic fatty liver
10.3760/cma.i.issn.0254-9026.2010.11.019
- VernacularTitle:己酮可可碱对非酒精性脂肪肝大鼠肝组织线粒体解耦联蛋白表达的影响
- Author:
Lishu XU
;
Yinghua ZHANG
- Publication Type:Journal Article
- Keywords:
Fatty liver;
Pentoxifylline proteins;
Mitochondrial;
Gene expression
- From:
Chinese Journal of Geriatrics
2010;29(11):937-939
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pentoxifylline (PTX) on the expression of uncoupling protein 2 (UCP-2) of hepatic mitochondria in rats with nonalcoholic fatty liver disease (NAFLD). Methods Sixty SD rats were randomly divided into 3 groups (each n = 20): normal control group, experiment group and treatment group. The rats in normal control group were given a normal feed. The rats in experiment and treatment groups were given fat-rich feed. Furthermore, the rats in treatment group were given PTX after 4 weeks in fat-rich diet feeding. The expression of UCP-2 in the liver was detected by immunohistochemistry and semi-quantitative RT-PCR. Results The hepatic expression of UCP2 mRNA was higher in experiment group (4.0±0.3) than in normal control group (1.2±0.1). The hepatic expression of UCP2 mRNA was higher in treatment group (3.0±0.2) than in normal control group, but the hepatic expression of UCP2 mRNA was lower in treatment group than in experiment group (F = 160. 67, P＜ 0. 01). Conclusions The UCP-2 mRNA is expressed in livers of NAFLD, pentoxifylline plays an important role in the reduction of expression of UCP-2 mRNA in lives of NAFLD.
