Effect of CHRNA1 genetic polymorphism on neuromuscular blockade induced with rocuronium
10.3760/cma.j.issn.0254-1416.2010.08.005
- VernacularTitle:CHRNA1基因多态性对罗库溴铵肌松效应的影响
- Author:
Yuanyuan GU
;
Yunshui PENG
;
Zixian SONG
;
Huiqun JIA
- Publication Type:Journal Article
- Keywords:
Polymorphism,single nucleotide;
Androstanols;
Neuromuscular blockade
- From:Chinese Journal of Anesthesiology
2010;30(8):910-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CHRNA1 genetic polymorphism on neuromuscular blockade induced with rocuronium. Methods Ninety-five ASA Ⅰ or Ⅱ patients of both sexes (age 18-64 yr,BMI 18-25 kg/m2 ) undergoing elective intra-abdominal surgery under general anesthesia were divided into 3 groups according to their genotypes: group Ⅰ AA ( n = 71 ); group Ⅱ AG ( n = 19) and group Ⅲ GG ( n = 5). ECG,BP, HR and SpO2 were continuously monitored during anesthesia. Neuromuscular function was assessed by response of adductor pollicis muscle to stimulation of the ulnar nerve using TOF-Watch SX monitor. Genomic DNA was extracted by using proteinase K digestion followed by a salting out prosedure. rs16862847 polymorphisms were analyzed by PCR-restriction fragment length polymorphism analysis and direct sequence analysis. Anesthesia was induced with fentanyl 4 μg/kg and propofol 2 mg/kg. Rocuronium 0.2 mg/kg was injected iv as soon as the patients lost consciousness. Results The twitch height of adductor pollicis muscle was significantly decreased in group AG and GG as compared with group AA ( P ＜ 0.05). There was no significant difference between group AG and GG.Conclusion CHRNA1 genetic polymorphism can influence the neuromuscular blockade induced with rocuronium,indicating that the genetic factor is one of the reasons contributing to the individual variation in neuromuscular blockade induced with muscle relaxants in patients.
