Effect of L-N6-(1-tminoethyl) Lysine on ischemia-reperfusion injury in a rat model of lung transplantation
10.3760/cma.j.issn.0254-1416.2010.08.024
- VernacularTitle:L-N6-(1-亚氨乙基)赖氨酸对大鼠移植肺缺血再灌注损伤的影响
- Author:
Hongwei ZHU
;
Jingxiang WU
;
Meiying XU
- Publication Type:Journal Article
- Keywords:
Lung transplantation;
Reperfusion injury;
L-N6-(1-Iminoethyl)Lysine
- From:
Chinese Journal of Anesthesiology
2010;30(8):973-975
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of L-N6-(1-iminoethyl) Lysine(L-NIL) on ischemia-reperfusion (I/R) -induced lung injury in a rat model of lung transplantation. Methods Pathogen free male SD rats weighing 250-350g were used as donor and recipient rats in this study. The animals were randomly divided into 3groups (n = 6 each): sham operation group (group S); lung tratsplantation group (group L) and lung transplantation + L-NIL (selective iNOS inhibitor) group (group L-NIL). In group L and L-NIL orthotopic left lung allograft transplantation was performed. In group L-NIL 3 mg/kg was injected iv at the beginning of reperfusion. The donor lungs were removed from live donor rats and placed in Euro-collins solution at 4 ℃. The lung transplantation was performed under microscope and non-suture cuff technique was used. The implanted donor lungs were ventilated and reperfused. 0.5% Evans blue 0.2 ml was injected iv during reperfusion. The donor lungs were removed after being implanted, ventilated and reperfused for 2 h for microscopic examination and determination of iNOS, endothelial NOS (eNOS) and myeloperoxidase (MPO) activity and malondialdehyde (MDA) and Evans blue content in the lung tissue and W/D lung weight ratio. Results Lung transplantation significantly inceased W/D ratio, iNOS and MPO activity, and Evans blue and MDA content in the lung tissue and decreased eNOS activity in group L as compared with group S. L-NIL iv significantly attenuated the increase in the variables mentioned above and ameliorated capillary congestion and inflammatory cell infiltration in the lung. Conclusion Intravenous L-NIL administered at the beginning of reperfusion can reduce I/R injury to the transplanted donor lungs.
