Role of NF-κB in ketamine-induced apoptosis in rat cortical neurons in vitro

VernacularTitle:NF-κB在氯胺酮诱导大鼠原代培养大脑皮层神经元凋亡中的作用
Author: Xian WANG ; Zhiqiang ZHOU ; Jianjun YANG ; Jianguo XU
Publication Type:Journal Article
Keywords: NF-kappa B; Ketamine; Apoptosis
From: Chinese Journal of Anesthesiology 2010;30(7):847-849
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of NF-κB in ketamine-induced apoptosis in rat cortical neurons in vitro. Methods Cortical neurons were isolated from newborn pathogen-free SD rats according to the method described by Kimney. After being cultured for 6 days the cortical neurons were randomly divided into 3 groups:group Ⅰ control (C); group Ⅱ ketamine (K) and group Ⅲ ketamine + SN50 (NF-κB translocation inhibitor) (KS). The cortical neurons were exposed to ketamine l mmol/L and ketamine 1 mmol/L + SN502.5 μmol/L for 24 h in group K and KS respectively. Twenty-four hours after the drugs were washed out, NF-κB activity, the expression of Bcl-XL and Bax and apoptosis in the neurons were measured. The ultrastructure of the neurons were examined by electron microscopy. Results NF-κB activity and the percentage of apoptotic neurons were significantly higher and Bcl-XL/Bax ratio was significantly lower in group K than in group C. There was no significant difference in NF-κB activity, Bcl-XL/Bax ratio and percentage of apoptotic neurons between group C and KS. Conclusion NF-κB is involved in ketamine-induced apoptosis in contical neurons in vitro.