Experimental study of the prevention and treatment of acute renal tubular necrosis with erythropoietin in rats
10.3760/cma.j.issn.1671-0282.2010.11.018
- VernacularTitle:促红细胞生成素防治大鼠急性肾小管坏死的实验研究
- Author:
Tanghong YU
;
Hong HU
;
Wei LIANG
;
Jinhong LIU
- Publication Type:Journal Article
- Keywords:
Erythropoietin;
Acute tubular necrosis;
Oxygen free radicals
- From:
Chinese Journal of Emergency Medicine
2010;19(11):1185-1187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the preventive effects of erythropoietin (EPO) on acute tubular necrosis of kidney in rats. Method The rat models of acute renal tubular necrosis were established with injecting glycerol in dose of 10 mL/kg. Thirty Wistar rats were randomly (random number) divided into control group, model group and EPO treatment group. EPO was administered intravenously into rats of treatment group in a dose of 1000IU/kg. Levels of blood urea nitrogen (BUN) and serum creatinine (Scr), urine osmolality, urine N-acetyl-D-glucosaminidase (NAG), urine β2-microglobulin (β2-MG), tissue MDA and SOD of rats in the three groups were assayed after the experiment. Renal histological examination was also performed. Results Compared with model group, the levels of BUN and Scr, urine osmolality, NAG,β2-MG and tissue MDA in EPO treament group were significantly lower, but urine osmolality and tissue SOD of rats remarkably increased in comparison with model group. EPO also lessened the histological changes in treatment group. Conclusions EPO has some protective effects on acute renal tubular necrosis in rats, which is probably through preventing oxygen free radical damage and elevating endogenous antioxidation potential.
