Effect of drynaria total flavonoid on TGF-β1 and BMP-2 secretion in the process of rats bone mesenchymal stem cells osteogenic differentiation
10.3760/cma.j.issn.1000-6699.2010.11.019
- VernacularTitle:骨碎补总黄酮对大鼠骨髓间充质干细胞成骨分化过程中TGF-β1、BMP-2分泌的影响
- Author:
Xiaochun SHU
;
Danhua ZHU
;
Hongyun LU
;
Tianjiao PANG
;
Liao SUN
;
Lihong YE
;
Jing SHEN
;
Danhong XIE
- Publication Type:Journal Article
- Keywords:
Bone mesenchymal stem cell;
Osteoporosis;
Osteogenic differentiation
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(11):990-992
- CountryChina
- Language:Chinese
-
Abstract:
The rats were assigned to blank control group, classical induction group, and drynaria total flavonoid group. Whole bone marrow culture method was applied to isolate and purify rats bone mesenchymal stem cells (BMSCs). Akaline phosphatase activity, calcium nodes, TGF-β1 and BMP-2 secretion in the process of bone mesenchymal stem cells osteogenic differentiation were detected. The results showed that compared to the blank group and classical group, drynaria total flavonoid promoted osteogenic differentiation accompanied with increased TGF-β1 and BMP-2 secretion (all P＜0. 05). Drynaria total flavonoid may promote osteogenic differentiation of BMSCs via upregulating TGF-β1 and BMP-2 expressions, and play an active role in the treatment of osteoporosis.
