Detection of Nanobacteria in Patients with Chronic Prostatitis and Vaginitis by Reverse Transcriptase Polymerase Chain Reaction.
10.4111/kju.2011.52.3.194
- Author:
Tae Hyoung KIM
1
;
Hye Ryoun KIM
;
Soon Chul MYUNG
Author Information
1. Department of Urology, Chung-Ang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Nanoparticles;
Prostatitis;
Reverse transcriptase polymerase chain reaction;
Vaginitis
- MeSH:
Calcifying Nanoparticles;
Chlamydia trachomatis;
Coinfection;
DNA;
Female;
Gonorrhea;
Humans;
Male;
Mycoplasma;
Mycoplasma hominis;
Nanoparticles;
Neisseria;
Pelvic Pain;
Prevalence;
Prostatitis;
Reproductive Tract Infections;
Reverse Transcriptase Polymerase Chain Reaction;
RNA-Directed DNA Polymerase;
Sexually Transmitted Diseases;
Trichomonas vaginalis;
Ureaplasma urealyticum;
Vaginitis
- From:Korean Journal of Urology
2011;52(3):194-199
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We aimed to investigate the detection of nanobacteria (NB) from expressed prostatic secretions (EPS) in patients with category III chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and from vaginal swabs in patients with vaginitis by reverse transcriptase polymerase chain reaction (RT-PCR) and to evaluate the association between NB and Neisseria gonorrhea, Chlamydia trachomatis, Ureaplasma urealyticum (U. urealyticum), Mycoplasma hominis, Trichomonas vaginalis, and Mycoplasma genitalium. MATERIALS AND METHODS: A group of 11 men attending a specialized CP/CPPS clinic and a group of 157 women who reported symptoms of lower genital tract infection were enrolled in this study. NB were detected by RT-PCR. A Seeplex Sexually Transmitted Disease Detection assay (Seegene Inc., Seoul, Korea) was used that could detect DNA for 6 types of sexually transmitted pathogens. RESULTS: In EPS samples, the detection rate of NB in patients with CP/CPPS was 9.1%, and 9 (5.7%) of 157 vaginitis patients showed positive results in RT-PCR for NB in vaginal swabs. Associations observed among the 7 microorganisms included 6 (54.5%) patients who tested positive on EPS and 75 (47.8%) patients who tested positive on vaginal swabs. Five patients with vaginitis were found to have monoinfection of NB (6.7%). CONCLUSIONS: We found that conventional RT-PCR for NB was rapid, simple, low in cost, and easily available for the detection of NB, and that NB may be a possible etiological factor for vaginitis and CP/CPPS. The prevalence of U. urealyticum among the four patients with NB coinfection was 75%; the presence of U. urealyticum might therefore raise suspicion for nanobacterial infection.
