Study of UTMD associated with shRNA techniques to silence Survivin expression and induce cell apoptosis

VernacularTitle:超声微泡靶向破坏转染shRNA沉默Survivin表达诱导裸鼠宫颈癌移植瘤凋亡的研究
Author: Zhiyi CHEN ; Kun LIANG ; Mingxing XIE
Publication Type:Journal Article
Keywords: Ultrasonography; Microbubbles; Gene therapy; Neoplasms; Apoptosis
From: Chinese Journal of Ultrasonography 2010;19(11):1001-1005
CountryChina
Language:Chinese
Abstract: Objective To investigate the gene silencing,apoptosis induction and the suppression of proliferation in vivo transfected by UTMD techniques associated with shRNA techniques. Methods The survivin-shRNA expression vector was constructed. Nude mice were randomly arranged into 3 groups:control group, plasmid injection and ultrasound (P + US), P + UTMD group. Histological examination were evaluated. Protein expressions of Survivin and proliferating cell nuclear antigen (PCNA), Bcl-2, Bax,Caspase-3, Ki-67, nucleostemin (NS), p53 were investigated by immunohistochemistry. Results In transplanted tumors experiment, comparing with those in C and P + US groups, protein expressions of PCNA,Ki-67,Bcl-2, Survivin, NS were down-regulated markedly, while those of Bax, Caspase-3 and P53 were up-regulated significantly ( P ＜ 0.05). Conclusions UTMD combined with shRNA technique can induce apoptosis and inhibit proliferation significantly, without causing any apparently adverse effect,representing a new,promising technology that can be used in the tumor gene therapy and research.