Association of IL-10-592A/C polymorphism with respiratory syncytial virus bronchiolitis
10.3760/cma.j.issn.1673-4912.2010.06.011
- VernacularTitle:呼吸道合胞病毒毛细支气管炎与白细胞介素-10-592A/C基因多态性的相关性研究
- Author:
Yu WANG
;
Wenxian CAI
;
Fenghai NIU
- Publication Type:Journal Article
- Keywords:
Respiratory syncytial virus;
Bronchiolitis;
Interleukin-10;
Gene polymorphism
- From:
Chinese Pediatric Emergency Medicine
2010;17(6):511-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association of IL-10-592A/C polymorphism with the susceptibility to respiratory syncytial virus(RSV) bronchiolitis and disease severity,and to explore the impact of IL10-592A/C polymorphism upon total serum IL-10 levels in children with RSV bronchiolitis. Methods One hundred children (patients group) admitted to hospital with RSV bronchiolitis and 100 healthy children (control group) were recruited. PCR restriction length fragment polymorphism assay was used to detect the single nucleotide polymorphisms of IL-10-592A/C. Total serum IL-10 levels in patients group were assayed with ELISA. Results The genotype frequency of IL-10-592A/C were AA 44% ,AC 38% ,CC 18% in patients group ,and AA 41% ,AC 42% ,CC 17% in the control group,it showed no significant difference between two groups (χ2= 0. 33, P > 0. 05) . The frequency of each allele were A 63%, C 37 % in patient group, and A 64% ,C 36% in the control group,there was no significant difference in allele frequency between two groups (χ2=0. 43 ,P >0. 05) . There was no significant difference in total serum IL-10 levels between different genotype of IL-10-592A/C (F = 0. 87, P > 0. 05) . The differences in genotype frequency of IL-10-592A/C were not significant between mild and moderate to severe cases (χ2= 2. 67, P > 0. 05) . Conclusion IL-10-592A/C gene polymorphisms do not correlated with RSV bronchiolitis.
