Detection of anti-cyclic citrullinated peptide antibodies by time-resolved fluoroimmunoassay
10.3760/cma.j.issn.1007-7480.2010.12.005
- VernacularTitle:时间分辨荧光免疫分析法测定抗环瓜氨酸肽抗体方法学的建立
- Author:
Mei LI
;
Guoqian CHEN
;
Yan YE
;
Yaohong ZOU
;
Ying ZHOU
;
Lei YU
;
Zhigang HU
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Anti-cyclic citrullinated peptide antibodies;
Time-resolved fluoroimmunoassay
- From:
Chinese Journal of Rheumatology
2010;14(12):815-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for detecting anti-cyclic citrullinated peptide (anti-CCP)in the serum of rheumatoid arthritis patients. Methods The range of lineage correlation, precision and detection range of time-resolved fluoroimmunoassay (TRFIA) was analyzed. Thirty-two positive serum of antiCCP, and the sera from 50 health blood donors, 32 SLE patients, 26 patients with SS, 10 patients with scleroderma, 20 patients with MCTD and 18 patients with MS were tested in this study. The clinical specificity was assessed. The consistency between TRFIA and ELISA was analyzed by Mc Nemar test. Results Analyzed by SPSS 13.0 statistical software, the intra-batch precision (n=20) rate was 2%, 3% and 4% respectively, and the inter-batch precision (n=8) was 3%, 4% and 7% respectively for 3 different concentrations. The clinical specificity was 98%, 97%, 96%, 100%, 95% and 100% in the sera of healthy blood donors, SL,E, SS,scleroderma, MCTD and MS patients respectively. The correlation coefficient was 0.989.The average recovery rate was 101%, and the sensitivity was 1 U/ml. When compared with ELISA, the detection range of TRFIA was wider and also with betterstability. Conclusion TRFIA is a stable method with high sensitivity and wide detection range. It can be used to detect anti-CCP antibody. It is important for early diagnosis of RA and monitor the curative effect of RA. And this method has potential to be used in clinical diagnosis.
