The study of tumor necrosis factor-alpla single nucleotide polymorphism in Chinese Han patients with polymyositis/dermatomyositis
10.3760/cma.j.issn.1007-7480.2010.12.007
- VernacularTitle:中国华东地区多发性肌炎/皮肌炎患者肿瘤坏死因子-α基因多态性研究
- Author:
Xiang GAO
;
Ling Lü
- Publication Type:Journal Article
- Keywords:
Dermatomyositis;
Tumor necrosis factor-alpha;
Polymorphism,single nucleotide
- From:
Chinese Journal of Rheumatology
2010;14(12):822-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the tumor necrosis factor alpha gene single nucleotide polymorphism (SNP) in Chinese Han polymyositis/dennatomyositis (PM/DM) patients. Methods A casecontrol study of three TNF-α SNPs were undertaken and comparison between cases of PM/DM (n=69) and DM patients ( n=52 ) with normal subjects ( n=57 ) was performed. The genotype of every subject was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results There was no significant difference in the genotypes and alleles frequencies of the three TNF-α SNPs(-238,-308, and -1031)between PM/DM or DM and normal subjects. The haplotype AGT (-238, -308, -1031 ) significantly increased in controls vs PM/DM and DM (PM/DM, P=0.01; DM, P=0.02) subjects. The haplotype AGC was a significant risk factor for DM (P=0.04, 0R=9.84, 95%CI 1.39～69.57 ). The haplotype AGC increased in PM/DM (4.3%)vs controls(0.6% ), but the difference was not significant (P=0.09, OR 7.22, 95%CI 1.02～50.90). The haplotype GGC was significantly decreased in the DM subgroup vs healthy control subjects (P=0.04, OR=0.47,95%CI 0.23～0.99). Conclusion The TNF gene (-238,-308,-1031) haplotype AGC is a risk factor for DM.
