The immune function influence of the CD4 + T helper cell after the treatment of continuous blood purification in a porcine model with multiple organ dysfunction syndrome
10.3760/cma.j.issn.1673-4912.2010.06.017
- VernacularTitle:连续性血液净化对多器官功能障碍幼猪T辅助细胞免疫功能影响的机制研究
- Author:
Jian ZHANG
;
Li ZHAO
;
Juan QIAN
;
Yanwen YANG
;
Hong REN
;
Xiaowei HU
;
Ying WANG
;
Biru LI
- Publication Type:Journal Article
- Keywords:
MODS;
Th1/Th2;
Continuous blood purification;
Porcine model
- From:
Chinese Pediatric Emergency Medicine
2010;17(6):522-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the levels of Th1 and Th2 cell in the peripheral and the cytokines (IFN-γ,TNF-α,IL-12,IL-10,IL-4) in the culture medium at different time in the porcine model with multiple organ dysfunction syndrome(MODS) after continuous blood purification(CBP). Methods Twenty-four young porcines were given intravenous infusion of endotoxin to induce MODS, then were randomly divided into 2 groups:CBP group(n = 12) and MODS group without CBP(n = 12). Continuous venovenous hemodiafiltration (CVVHDF) was the main mode for CBP. At baseline, onset of MODS, and 2 h,4 h,6 h after treatment of CVVHDF,we picked the blood respectively to separate the T helper cell using the beads,detected the Th1 and Th2 cell by FACS and detected the cytokines (IFN-γ,TNF-α,IL-12,IL-10,IL-4) in the culture medium by ELISA. Results The MODS group showed a obvious rise in TNF-α,the drop tendency in IL-12,IFN-γ,IL-10,Th1/Th2 ratio, and no significant change in IL-4. After the treatment, the CBP group showed the drop in TNF-α and IL-4;and increase in IL-12,IFN-γ,IL-10 and Th1/Th2 ratio. Conclusion CBP helps to dear immune function,and restore the balance of Th1/Th2 in porcine MODS model.
