Clinical significance of chemokine CCL20 and its receptor CCR6 expression in patients with gastric cancer
10.3760/cma.j.issn.1673-4203.2010.12.009
- VernacularTitle:胃癌患者血清中CCL20和CCR6的表达及临床意义
- Author:
Zhigang LIU
;
Danfeng LIU
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Chemokine CCL20 CCR6;
Flow Cytometry
- From:
International Journal of Surgery
2010;37(12):816-819
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of CCL20 and CCR6 in the patients with gastric cancer and To examine the relationship between chemokine expression and the occurrence and development of Gastric Cancer. Methods Real-time PCR , flow cytometry and ELISA are used to measure the gene transcription and protein expression levels of chemokine CCL20 and CCR6 in the serum of 50 patients with Gastric Cancer and 30 normal controls. Results The gene expression levels CCL20 and CCR6 in Gastric Cancer group are significantly higher than that in healthy controls. The level protein of CCL20 and CCR6 in peripheral blood of patients with gastric cancer are significantly higher than that in healthy peep le[ (45.4 ±10.9) pg/mL vs (18.6±4.7) pg/mL; (7.11 ±1.03%) vs (1.83±0.43%), P＜0.01. respectively],and the increase significantlyassociated with the clinical stage of Gastric Cancer. Conclusions The method for detecting the expression of CCL20 and CCR6 in patient with Gastric Cancer has been successfully established, and their expression levels were found to be correlated with the occurrence and development of Gastric Cancer. Thus, CCL20 and CCR6 may be involved in the regulatory mechanisms associated with the development of Gastric Cancer, and may be valuable in its diagnosis and prevention.
