Effects of radon and its progeny on the expression and mutation of p53 in lung tissues of mice

VernacularTitle:氡及其子体暴露小鼠肺组织p53基因表达及突变研究
Author: Chunnan PIAO ; Mei TIAN ; Jianxiang LIU ; Jianlei RUAN ; Xu SU
Publication Type:Journal Article
Keywords: Radon and its progeny; Lung injury; Apoptosis; p53; Mutation
From: Chinese Journal of Radiological Medicine and Protection 2010;30(6):696-700
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of radon and its progeny on the expression and mutations of p53 in lung tissue of mouse model. Methods Apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling. The expression of p53 gene was analyzed by immunohistochemistry, Western blot and realtime-PCR. PCR-SSCP was used to detect the mutation of p53 in lung tissues. Results Compared with those in the control group, the apoptotic index were increased significantly in 30 WLM and 60 WLM groups( t = 18.11, -10.30,P ＜ 0.05 ). The p53 protein was increased significantly ( t = -11.08, P ＜ 0.05; t = - 7.00, P ＜ 0.0 ) ) in 30 WLM and 60 WLM groups. The mutation of p53 gene was not detected in lungs of radon-exposure mice. Conclusions Lung and bronchus might be the targets of radon and its progeny, and p53 gene plays an important role in the progression of radon-induced lung injury.