The preparation of targeted microbubble with low immunogenicity
10.3760/cma.j.issn.1004-4477.2010.12.023
- VernacularTitle:低免疫原性靶向微泡的制备研究
- Author:
Guangliang HUANG
;
Wei WANG
;
Xiaoyan XIE
;
Huixiong XU
;
Zuofeng XU
;
Guanjian LIU
;
Mingde Lü
- Publication Type:Journal Article
- Keywords:
Microbubbles;
Immunocompetence;
Biological markers
- From:
Chinese Journal of Ultrasonography
2010;19(12):1079-1081
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare targeted micorbubble with low immunogenicity. Methods The microbubbles were produced with different phospholipids and identified by the fluorescent method. Detect the level of C3a after reaction with human serum in vitro with enzyme-linked immunosorboent assay (ELISA) method and the number of microbubble binding with the streptavidin packed on the dish by using the parallel plate flow chamber. Results The level of C3a was (1.037±0.047)ng/ml in MBb group,(1. 326 ± 0. 042)ng/ml in MBe group and ( 1.004 ± 0.031 ) ng/ml in MBc group. The level of C3a in MBb group was significantly lower than that in MBe group( P ＜0.05),and there was no significantly difference between MBb group and MBc group ( P ＞ 0. 05). The parallel plate flow experiments showed that the number of MBb(15.2 ± 11.3) in each field of view binding with the streptavidin packed on the dish was significantly fewer than that of MBe ( 103.2 ± 28.3) ( P＜0.05 ), and there was no significantly difference between MBb and MBc(17.8 ± 11.9) ( P ＞0.05). Conclusions The targeted microbubble with low immunogenicity has been prepared successfully,which can be used for further experiment in vivo.
