Association of CA repeats polymorphism in the promoter region of the insulin-like growth factor- Ⅰ gene with type 2 diabetes

VernacularTitle:胰岛素样生长因子Ⅰ基因启动子区CA重复序列多态性与2型糖尿病的相关性
Author: Min YANG ; Wei LING ; Jing LIN ; Changlin NI
Publication Type:Journal Article
Keywords: Insulin-like growth factor-Ⅰ; Polymorphism; Diabetes mellitus,type 2; Uric acid
From: Chinese Journal of Endocrinology and Metabolism 2010;26(12):1042-1044
CountryChina
Language:Chinese
Abstract: Objective To investigate the association of CA repeats polymorphism in the promoter region of human IGF-I gene with type 2 diabetes in narthen Han nationality. Methods Gender-age matched subjects with type 2 diabetes and normal glucose tolerance (NGT) were enrolled for this study, 147 subjects in type 2 diabetes group and 159 subjects in NGT group. Genomic DNA was extracted by standard methods. PCR, Genescan,Genotyper, and direct sequencing were conducted to screen CA repeats polymorphism in the promoter region of the human IGF-I gene. Results No significant association was observed between any ( CA), repeat genotype and type 2 diabetes. A lowered serum uric acid was seen in genotypes that included alleles with larger than (CA) 20 repeats [(4.18 ± 1.25 vs 4.63 ± 1.36) mg/dl, P = 0.03]. Conclusion Alleles with larger than (CA) 20 repeats may be a protective factor for type 2 diabetes.