Preliminary study on high mobility group box 1 as a biological dosimeter of ionizing radiation

VernacularTitle:高迁移率族蛋白B1作为电离辐射生物剂量计的初探
Author: Yang JIAO ; Wei ZHU ; Lili WANG ; Yusong ZHANG ; Jiaying XU ; Saijun FAN
Publication Type:Journal Article
Keywords: HMGB1; Ionizing irradiation; Biological dosimeter
From: Chinese Journal of Radiological Medicine and Protection 2010;30(6):647-649
CountryChina
Language:Chinese
Abstract: Objective To investigate the probability of high mobility group box 1 protein as a biological dosimeter of ionizing radiation. Methods The medium of cultured human fibroblast cell line GM was collected 24 h after exposed to 60Co γ-rays, and HMGB1 protein concentration was detected by ELISA assay, and the dose-effect curve was then fitted. The levels of HMGB1 protein was also detected after exposed to 4 Gy irradiation at 24,48 and 72 h. Results The level of HMGB1 protein in culture medium was increased in a dose-dependant way, and the dose-effect curve of HMGB1 level for 24 h after irradiation fitted the linear model y = 0.5655 + 0.0358x (r = 0. 9339) . After exposure to 4 Gy irradiation, the levels of HMGB1 protein in the culture medium were also increased time-dependently.Conclusion The release of HMGB1 protein to neoplasm is reactivated by irradiation in GM cell. It is necessary to further the studies on HMGB1 as biological dosimeter for radiation injury protection and therapy.