Study of antisense oligonucleotide miR-21 on radiosensitivity of SHG-44 in vitro
10.3760/cma.j.issn.0254-5098.2010.06.017
- VernacularTitle:miR-21反义寡核苷酸对SHG-44放射增敏作用的体外研究
- Author:
Chong ZHOU
;
Juying ZHOU
;
Lili WANG
;
Zhiying YU
;
Xiaoting XU
;
Songbing QIN
;
Bin NIE
- Publication Type:Journal Article
- Keywords:
Glioma;
Radiosensitivity;
miR-21;
Cell Cycle;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(6):701-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the radiosensitizing effect of knock-down the expression of miR-21 on human SHG-44 glioma cells and explore the possible mechanism. Methods Antisense oligonuleotidas of miR-21, mediated by LipofectamineTM 2000, were transfected to SHG-44 cells. Three groups were: blank control group ( mock group), negative control and antisense transfected group ( AS-miR-21 gorup). Cells of each group were irradiated with 6 MeV X-rays at the doses of 0,1,2,4,6 and 8 Gy.Dose-suvivial curve was established by colony-forming assay. The influence of AS-miR-21 on cell cycle and cell apoptosis was analyzed by flow cytometry assay after 6 Gy irradiation. Results The value of D0 and Dq of AS-miR-21 group declined obviously compared with the mock group and negative control group. Flow cytometric analysis showed that cell cycle distribution changed( G0/G1 phase arrest, S phase decreased)after transfected with AS-miR-21 (t = 8.18, -4.52,P ＜ 0.05 ). The sensitization enhancement ratios of D0 and Dq were 1.32 and 2.10 respectively. Apoptosis assay showed the early apoptosis rate was signiflcantely increased in AS-miR-21 、irradition alone and combined group than mock control group( t = 20.14,11.11,50.07, P ＜ 0.05). Conclusions AS-miR-21 can enhance the radiosensitivity of human glioma cells SHG-44 by promoting cell apoptosis and faciliating cell cycle redistribution.
