Effect of penehyclidine hydrochloride on brain injury induced by cardiopulmonary bypass in rats
10.3760/cma.j.issn.0254-1416.2010.09.018
- VernacularTitle:盐酸戊乙奎醚对体外循环致大鼠脑损伤的影响
- Author:
Huijuan CAO
;
Jin ZHOU
;
Yingjie SUN
;
Tiezheng ZHANG
;
Jing YAO
- Publication Type:Journal Article
- Keywords:
Cholinergic antagonists;
Cardiopulmonary bypass;
Brain
- From:
Chinese Journal of Anesthesiology
2010;30(9):1081-1084
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of penehyclidine hydrochloride on brain injury induced by cardiopulmonary bypass (CPB) in rats. Methods Thirty adult male SD rats were randomly divided into 5 groups ( n = 6 each): sham operation group (group S), CPB group, and low, median and high dose penehyclidine hydrochloride groups (group PL, PM , PH). Penehyclidine hydrochloride 0.2, 0.6 and 2.0 mg/kg were added to the priming solution in group PL, PM and PH respectively, while the equal volume of normal saline was added instead in group S. Blood samples were obtained at 2 h after termination of CPB to determine the plasma concentrations of neuron specific enolase (NSE) and S-100β protein. The brain tissues were taken to observe the ultrastructure of hippocampal neurons with electron microscope. Results The concentrations of NSE and S-100β protein were significantly higher in the other groups than in group S, while lower in group PM and PH than in group CPB and PL( P＜ 0.05). The S-100β protein concentration was significantly lower in group PH than in group PM( P ＜ 0.05). The damage to hippocampal neurons was significantly attenuated in group PM and Ps. Conclusion Penehyclidine hydrochloride 0.6 or 2.0 mg/kg can reduce the CPB-induced brain injury in a dose-dependent manner in rats.
