Effects of glucose on apoptosis of endothelial progenitor cells from human peripheral blood

VernacularTitle:葡萄糖对人外周血内皮祖细胞凋亡的影响
Author: Lin LI ; Minxiang LEI ; Hangsong XU ; Zehao LIU
Publication Type:Journal Article
Keywords: Glucose/PD; Endothelial cells/DE/PA; Apoptosis/DE
From: Journal of Chinese Physician 2010;12(12):1620-1622
CountryChina
Language:Chinese
Abstract: Objectives To investigate the effects of glucose on apoptosis rate of cultured endothelial progenitor cells (cEPCs). Methods The peripheral blood of healthy adults was isolated by density gradient centrifugation, and mononuclear cells (MNCs) were inducted to differentiate at cultured conditions.EPCs were identified by Dil-acLDL and FITC-UEA-1 as double fluorescent-positive cells. The effectsof glucose at different concentrations on apoptosis rate of the harvested EPCs were measured by fluorescent microscope and fluorescence-activated cell sorting (FACS) after staining with Annexin V-FITC and PI. Results No significant differences were observed in apoptosis rate between samples treatedwith 5.6mmol/l glucose and 11. 1 mmol/l. P ＞0. 05). 25.5 mmol/L glucose enhanced the EPCsapoptosis rate in a time-dependent manner( P ＜0. 05). Conclusion High concentration glucose can accelerate apoptosis rate of EPCs in a time-dependent manner.