Effects of Netrin-1 on angiogenesis in vitro and in vivo
10.3760/cma.j.issn.1008-6315.2010.12.013
- VernacularTitle:轴突导向因子-1在血管生成模型中的作用研究
- Author:
Qianhua WANG
;
Jianwen ZHU
;
Shaoping ZHONG
;
Li ZOU
- Publication Type:Journal Article
- Keywords:
Netrin-1;
Matrigel plug assay;
Angiogenesis;
Aortic ring
- From:
Clinical Medicine of China
2010;26(12):1265-1267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Netrin-1 on angiogenesis in vitro and in vivo. Methods We performed in vitro rat aortic ring assay and in vivo Matrigel plug assay to determine the effect of Netrin-1 on angiogenesis. Results 10 μg/L, 50 μg/L and 100 μg/L Netrin-1 stimulated microvessel sprouting from the adventitia of aortic rings and the effect reached maximum at 50 μg/L The in vivo Matrigel plug assay showed orange color change if Nestrin-1 was positive; and CD34 immunofluorescent staining showed vascular structures in the Matrigel plug with hemachrome ( 53.4 ± 7. 3 ), which was significantly higher than control ( 5. 8 ± 0. 9 )Conclusions Netrin-1 can induce angiogenesis in vitro and in vivo.
