Ultrasound-mediated destruction of microbubbles directs SERCA2a gene delivery to rats with myocardial infarction by two-dimensional ultrasound speckle tracking imaging
10.3760/cma.j.issn.1004-4477.2010.12.021
- VernacularTitle:超声斑点追踪技术评价微泡造影剂介导SERCA2a基因治疗心肌梗死大鼠的实验研究
- Author:
Xinkui JIANG
;
Yuming MU
;
Chunmei WANG
;
Qi TANG
;
Lina GUAN
- Publication Type:Journal Article
- Keywords:
Echocardiography;
Microbubbles;
Myocardial infarction;
Ventricular function,left;
Gene therapy
- From:
Chinese Journal of Ultrasonography
2010;19(12):1072-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the therapeutic effects of rAAV2-SERCA2a gene targeted to myocardium by the ultrasound mediated microbubbles in rats with myocardial infarction(MI) using speckle tracking echocardiography (STE). Methods Thirty-five male SD rats were divided into Sham group, MI group and gene therapy group. rAAV2-SERCA2a was attached to the surface of SonoVue, sulphur hexafluoride-filled microbubbles. Therapy group animals were infused with those mixed fluids through tail vein. The other two groups were infused with SonoVue microbubbles. These bubbles were infused with simultaneous echocardiography. Four weeks after ultrasound microbubbles destructed,Circumferential strain rate(SRcir), radial strain rate(SRrad) and left ventricular torsion (LVtor) of regional myocardium were measured in short-axis view using speckle tracking imaging software for each animal. Results Compared with the M1 group,there were significant increase in SRcir-S,SRcir-E, SRrad-S,SRrad-E and LVtor in rats of gene therapy group( P ＜0. 01 ). There was no significant difference between gene therapy group and sham group. Conclusions rAAV2-SERCA2a gene targeted to myocardium by the ultrasound mediated microbubbles could enhance cardiac function of rats with MI.
