Preparation of lipsomal Clodronate and its inducing effect on apoptosis of peritoneal macrophages in rats after severe acute pancreatitis
10.3760/cma.j.issn.1007-8118.2010.12.015
- VernacularTitle:Clodronate脂质体的制备及诱导重症急性胰腺炎大鼠腹腔巨噬细胞凋亡的实验研究
- Author:
Huazhong CAI
;
Jianxin ZHANG
;
Jiangtao YIN
;
Lei CUI
;
Shengchun DANG
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Peritoneal macrophage;
Apoptosis;
Liposomal clodronate
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(12):943-946
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preparation of liposomal clodronate and investigate its inducing effects on the apoptosis of peritoneal macrophages in rats after severe acute pancreatitis (SAP).Methods Liposomal clodronate was prepared by means of thin film. SAP rat model was established by retrograde injection of 5% sodium taurocholate into the pancreatic duct. The peritoneal macrophages were obtained from SAP rats. After exposure to different doses of liposomal clodronate (50, 100,150 μl), the PM proliferation was determined by MTT colourimetry. The apoptosis of PM was measured by flow cytometry and agarose gel electrophoresis, respectively. Results The prepared liposomal clodronate had a suitable encapsulation efficiency of clodronate (5.8%) with an average size of 200 nm. The spherical shape of liposome was confirmed by transmission electron microscope. Exposed to liposomal clodronate of different doses resulted in a obvious growth depression (P＜0.01). The apoptotic rate of the PM was (10.32±0.34) %, (18.16±0.49)% and (29.87±0.35)% in three different dose groups and the difference was marked (P＜0.01). 1.2% of agarose gel electrophoresis of DNA extracted from apoptotic macrophages induced by liposomal clodronate showed clearer and characteristic ladder following the liposomal clodronate concentration. Conclusion Liposomal clodronate has a definite effect on peritoneal macrophages in SAP rats.
