Effects of hydrogen-rich saline combined with mild hypothermia on cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2010.09.033
- VernacularTitle:富氢液联合浅低温对大鼠脑缺血再灌注损伤的影响
- Author:
Dan LI
;
Xiaolei MIAO
;
Junfang LI
;
Kangli HUI
;
Xiaodi SUN
;
Jian ZONG
;
Xuejun SUN
;
Manlin DUAN
;
Jianguo XU
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Hypothermia,induced;
Reperfusion injury;
Brain
- From:
Chinese Journal of Anesthesiology
2010;30(9):1126-1129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hydrogen-rich saline combined with mild hypothermia on cerebral ischemia-reperfusion (IR) injury in rats. Methods Fifty male SD rats, aged 9-10 weeks, weiging 250-300 g, were randomly divided into 5 groups (n= 10 each): sham operation group (group S), group IR, hygrogen-rich saline group (group H), mild hypothermia group (group M) and hydrogen-rich saline + mild hypothermia group (group HM). In group IR, H, M and HM cerebral IR was induced by 15 min ligation of bilateral carotid artery followed by 6 h reperfusion. In group H and HM intraperitoneal hydrogen-rich saline 5 ml/kg was injected immediately after reperfusion, while the equal volume of normal saline was injected instead of hydrogen-rich saline in the other three groups. At the same time, the rectal temperature was maintained at 37-38 ℃ in group S,IR and M, while it was reduced to 32-34 ℃ by physical method within 15 min, lasting for 6 h, in group M and HM. The animals were sacrificed after 6 h of reperfusion, and then the hippocampus was removed for microscopic examination. The expression of HO-1 and content of MDA and TNF-α were determined by Western blot. Results The cerebral IR injury was attenuated in group H, M and HM compared with group IR, with the slightest injury in group HM. The expression of HO-1 and content of MDA and TNF-α were significantly higher in group IR, H, M and HM than in group S. The expression of HO-1 was significantly higher, while the content of MDA and TNF-α were lower in group H, M and HM than in group IR, and in group HM than in group H and M. There was no significant difference in the expression of HO-1 and content of MDA and TNF-α between group H and M. Conclusion Hydrogen-rich saline combined with mild hypothermia can attenuate cerebral I/R injury in rats via up-regulating the expression of HO-1 and decreasing the content of MDA and TNF-α in hippocampus.
