Inhibition of proliferation of 5-FU combined with thermotherapy in human hepatoma cell line
10.3760/cma.j.issn.1008-6315.2010.12.001
- VernacularTitle:5-氟尿嘧啶联合热疗对人肝癌细胞株的增殖抑制作用
- Author:
Xianying LIU
;
Maofeng XU
;
Cunxiang JIN
;
Wei ZHANG
;
Xiangjun LI
;
Huihui YANG
;
Han SUN
- Publication Type:Journal Article
- Keywords:
Human hepatoma cell line;
5-FU;
Thermotherapy;
Cell cycle
- From:
Clinical Medicine of China
2010;26(12):1233-1235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition rate of cell proliferation, cell apoptosis rate and their effects on the cell cycle proceeding of the SSMC7721 cell line when 5-FU combined with thermotherapy is induced into the cells, and then provide theoretical bases to the combined therapy of hepatocellular carcinoma. Methods The inhibition rate of cell proliferation was detected by the MTT under different conditions, the cell cycle proceeding and the cell apoptosis rate was detected by flow cytometry and the subcellular structure was detected by the electronmicroscope. Results The cell inhibition rate of the thermotherapy group, 5-FU group and the combinedgroup were 18.4% ,28. 3% and 52. 7% ,respectively. The inhibition rates in the latter two groups were significantly different to the thermotherapy group. The results of flow cytometry showed that the cell numbers increased in G1 stage decreased in S stage,and increased in G2/M stage;the cell apoptosis rate increased. There was significant difference between different groups(P ＜ 0.01 or P ＜0.05). The results of the electronmicroscop showed that the nuclear chromatins agglutinated in the borderline and the mitochondriums became swelled. Conclusions The 5-FU combined with thermotherapy could significantly improve the inhibition rate of cell proliferation, inhibit the cell cycle proceeding from G1 stage to S stage, and induce cells apoptosis and change the subcellular structures in the SSMC7721 cell line.
