Effect of RNA interference on the bionomics of Cathepsin L of hepatoma carcinoma cells

VernacularTitle:RNA干扰沉默Cathepsin L基因表达对肝癌细胞生物学特性影响的实验研究
Author: Yun JIN ; Ding LUO ; Junhua AI ; Jiahong DONG
Publication Type:Journal Article
Keywords: Carcinoma hepatocellular; RNAi; Cathepsin L
From: Chinese Journal of Hepatobiliary Surgery 2010;16(12):931-934
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of RNA interference on the bionomics of Cathepsin L of hepatoma carcinoma cells. Methods In this study, the experimental group was the Cathepsin L RNA interference group. Control groups were the normal blank hepatoma carcinoma cell group (the blank group) and the Cathepsin LRNA interference blank group (the fluorescence control group). Observing times were ld,3d and 6d after RNA interference. Transfection efficiency in each group was observed. Expression of Cathepsin L of hepatoma cells was detected by immunofluorescence, RT-PCR and WB. Cell vigor was detected by MTT assay. Changes in the cell cycle and apoptosis were observed by flow cytometry. Transwell cabin was used to detect the changes of cell invasive power.Results In the experimental group, Cathepsin L mRNA level and protein level significantly decreased, the proliferation index significantly decreased and apoptosis index significantly increased. The invasive power also decreased. Conclusion RNAi interference can inhibit Cathepsin L expression, cell proliferation and cell invasive power efficiently.