Effects of etomidate preconditioning on expression of HL-60 cell apoptosis-related proteins
10.3760/cma.j.issn.0254-1416.2010.09.015
- VernacularTitle:依托咪酯预处理对HL-60细胞凋亡相关蛋白表达的影响
- Author:
Yuxia JIAO
;
Junyu XIONG
- Publication Type:Journal Article
- Keywords:
Etomidate;
Ischemic preconditioning;
Apoptosis;
Caspases
- From:
Chinese Journal of Anesthesiology
2010;30(9):1074-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of etomidate preconditioning on the expression of procaspase-3, caspase-9 p35 and caspase-8 p20 in HL-60 cells. Methods HL-60 cells were purchased from Shanghai life science institute and cultured in RPMI-1640 culture medium at 37℃ in 5% CO2 incubator. The cells were randomy divided into 3 groups ( n = 3 each): control group (group C), etomidate group (group E) and etomidate preconditioning group (group EP). In group E, the cells were exposed to 500 μmol/L etomidate and incubated for 24 h. In group EP, the cells were exposed to 1μmol/L etomidate for 1 h and was allowed to recover for4 h after etomidate washout, then etomidate 500μmol/L was added and the cells were incubated for 24 h. The expression of procaspase-3, caspase-9 p35 and caspase-8 p20 was determined using Western blot. Results The procaspase-3 expression was significantly down-regulated, while the expression of caspase-9 p35 and caspase-8 p20 was up-regulated ingroup E and EP as compared with group C ( P ＜ 0.05). The procaspase-3 expression was up-regulated,while the expression of caspase-9 p35 and caspase-8 p20 was down-regulated in group EP as compared with group E ( P ＜ 0.05). Conclusion Etomidate preconditiong can inhibit etomidate-induced down-regulation of procaspase3 expression and up-regulation of caspase-9 p35 and caspase-8 p20 expression, resulting in suppression of HL-60 cell apoptosis induced by etomidate.
