Effects of hemin on neuroglobin expression after cardiopulmonary resuscitation in rats
10.3760/cma.j.issn.1671-0282.2010.12.014
- VernacularTitle:心肺复苏后大鼠脑红蛋白变化及氯化血红素的作用
- Author:
Aiwen HE
;
Ting YANG
;
Shouquan CHEN
;
Zhangping LI
;
Huiping LI
;
Weijia HUANG
;
Junyan CHENG
;
Jie ZHANG
;
Ping YAN
;
Wantie WANG
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary resuscitation;
Neuroglobin;
Neurodeficit score;
IIemin;
Cerebral injury;
Rats
- From:
Chinese Journal of Emergency Medicine
2010;19(12):1287-1290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Hemin on the level of neuroglobin (NGB) in cerebral cortex,neurodeficit score (NDS) and pathological changes in cerebral cortex after cardiopulmonary resuscitation (CPR) in rats. Method A total of 120 male Sprague Dawley(SD) rats were divided randomly into control group(A), CPR group(B) and Hemin group(C). The animal model of cardiac arrest (CA) induced by asphyxia was established and CPR was performed. The NGB level in cerebral cortex, NDS and pathological changes in cerebral cortex were examined 0.5 h,3 h,6 h, 12 h, 24 h after restoration of spontaneous circulation (ROSC) in each group. Experimental data were analyzed by using one-factor analysis of variance and Tukey test. Results In comparison with group A, the levels of NGB were significantly higher 12 h,and 24 h after ROSC (P ＜0.05 and P ＜0.01), the values of NDS were significantly lower at each interval after ROSC ( P ＜ 0.01 ) ,and the pathological changes were more severe at each interval after ROSC in group B. In comparison with group A, the levels of NGB were significantly higher 6 h, 12 h and 24 h after ROSC ( P ＜ 0.05 and P ＜ 0.01 ), and the values of NDS were significantly lower 3 h, 6 h, 12 h after ROSC ( P ＜ 0.01) in group C. In comparison with group B, the levels of NGB were significantly higher 12 h and 24 h after ROSC, the values of NDS were significantly higher 12 h and 24 h after ROSC, and the pathological changes were less in group C. Conclusions The NGB level increased in cerebral cortex, the NDS level decreased and severe pathological changes occurred in rats after CPR. The hemin treatment up-regulated the level of NGB, improved the NDS, mitigated pathological changes, alleviating the cerebral injury after CPR.
