The open field test as a method for ethology in Kunming mice: test-retest reliability
10.3760/cma.j.issn.1674-6554.2010.12.014
- VernacularTitle:旷场实验:昆明小鼠行为学评价方法的重测信度检验
- Author:
Shiguang SUN
;
Jingjing WANG
;
Zifa LI
;
Ming HUANG
;
Jian LIU
;
Sheng WEI
;
Huiyun ZHANG
;
Mingqi QIAO
- Publication Type:Journal Article
- Keywords:
Open field test;
State anxiety;
Ethology;
Test-retest reliability;
Kunming Mice
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(12):1093-1095
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the test-retest reliability of the open field test,as a method for ethology in Kunming mice. Methods The behavior of adult male Kunming mice in the open field test was recorded twice,once for five minutes, with a one-week inter-trial interval. The following parameters were evaluated:number of central squares; number of peripheral squares; percent of central squares; number of horizontal squares; number of rearings; total score; and number of fecal boli. Subsequently,the intraclass correlation coefficient (ICC) and the agreement index (Kappa) were calculated for each of these parameters. Results Both ICC and Kappa for total score( ICC =0.902, P＜0.01; Kappa =0.680, P＜0.01; r=0.815, P＜0.01 ) showed a good reliability and correlation between the test trials. Only ICC for number of central squares ( ICC = 0. 578, P ＜ 0.01 ), number of peripheral squares( ICC =0.615, P＜0.01 ) ,percent of central squares(ICC=0.523, P＜0.01) ,number of horizontal squares( ICC = 0. 607, P ＜ 0.01 ) and number of rearings ( ICC = 0. 606, P ＜ 0.01 ) indicated a fair reliability between the test trials. Neither ICC nor Kappa for number of fecal boli displayed a significant reliability between the test trials. The difference of these parameters between test and retest, such as number of peripheral squares( t =3.01, P＜0.01 ),number of horizontal squares( t = 3.34, P ＜ 0.01 ) and total score( t = 7.86, P ＜ 0.01 ) were significant. Conclusion The parameter,total score for Kunming mice in the open field test,is found to be stable over time, and indicates a good reliability with a one-week inter-trial interval.
