Effect and mechanism of preptin on connective tissue growth factor expression in human osteoblasts
10.3760/cma.j.issn.1000-6699.2010.11.016
- VernacularTitle:Preptin对成骨细胞结缔组织生长因子表达的影响及其机制研究
- Author:
Huawen WANG
;
Youshuo LIU
;
Junkun ZHAN
;
Hui XIE
;
Xianping WU
;
Lingqing YUAN
;
Eryuan LIAO
- Publication Type:Journal Article
- Keywords:
Preptin;
Osteoblast;
Connect tissue growth factor;
Extracellular signal regulated kinase
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(11):978-980
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechnism of preptin on connect tissue growth factor (CTGF) in human osteoblasts. Methods Recombinant human preptin was used to treat primary human osteoblasts, and Western blot was used to detect CTGF protein level. Mitogen-activated protein kinase p38(p38MAPK), extracellular signal-regulated kinase (ERK1/2), c-jun N-terminal Kinase (JNK), and their phosphorylation levels were also detected by Western blot. MAPK inhibitors (PD98059, SP600125, or SB203580)were used to elucidate the mechnism of preptin induced expression of CTGF in human osteoblasts. Results Treatment of human osteoblasts with preptin caused a time and dose-dependent increase in CTGF secretion. Preptin induced activation of ERK, but not p38MAPK or JNK in human osteoblasts. Furhermore, pretreatment of human osteoblasts with the ERK inhibitor PD98059 abolished the preptin-induced CTGF secretion. Conclusion Preptin induces CTGF expression in human osteoblasts by means of ERK/MAPK pathway.