Effect and mechanism of preptin on connective tissue growth factor expression in human osteoblasts

VernacularTitle:Preptin对成骨细胞结缔组织生长因子表达的影响及其机制研究
Author: Huawen WANG ; Youshuo LIU ; Junkun ZHAN ; Hui XIE ; Xianping WU ; Lingqing YUAN ; Eryuan LIAO
Publication Type:Journal Article
Keywords: Preptin; Osteoblast; Connect tissue growth factor; Extracellular signal regulated kinase
From: Chinese Journal of Endocrinology and Metabolism 2010;26(11):978-980
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect and mechnism of preptin on connect tissue growth factor (CTGF) in human osteoblasts. Methods Recombinant human preptin was used to treat primary human osteoblasts, and Western blot was used to detect CTGF protein level. Mitogen-activated protein kinase p38(p38MAPK), extracellular signal-regulated kinase (ERK1/2), c-jun N-terminal Kinase (JNK), and their phosphorylation levels were also detected by Western blot. MAPK inhibitors (PD98059, SP600125, or SB203580)were used to elucidate the mechnism of preptin induced expression of CTGF in human osteoblasts. Results Treatment of human osteoblasts with preptin caused a time and dose-dependent increase in CTGF secretion. Preptin induced activation of ERK, but not p38MAPK or JNK in human osteoblasts. Furhermore, pretreatment of human osteoblasts with the ERK inhibitor PD98059 abolished the preptin-induced CTGF secretion. Conclusion Preptin induces CTGF expression in human osteoblasts by means of ERK/MAPK pathway.