Effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 on livability of SN56 cells
10.3760/cma.j.issn.1674-6554.2010.12.015
- VernacularTitle:低浓度Aβ1-42单体/寡聚体及一氧化碳供体对SN56细胞存活率的影响
- Author:
Man JIN
;
Peiyuan LU
;
Ling LI
;
Yanghong DONG
;
Hongjuan YUE
;
Jinling LI
;
Mingyue FAN
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Aβ1-42;
Carbon monoxide;
SN56 cells;
Livability
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2010;19(12):1096-1098
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 in different concentration on livability of SN56 cells. Methods SN56 cells were cultured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42 + CORM-2 100 μM group. Three lines of cells in Aβ1-42 group were cultured in the surroundings of 10nM,100nM and 1 μM Aβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same culture condition as group Aβ1-42 ,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the livability of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was (79.73 ±0.94)% ,(67.99 ±0.79)% ,(60.42 ±0.62)% , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 50μM/100μM was( 75.15±0.096)%,(63.20 ±0.17)%, (55.33 ±0.19)%; (73.20 ±0.27)%, (64.34 ±0.11 )%, (54.17 ±0.12)% , respectively. Both were lower than group Aβ1-42. And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livability of SN56 cells,and there is a discrepancy in the intensity.
